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KMID : 1037020180070020062
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Medical Lasers; Engineering, Basic Research, and Clinical Application
2018 Volume.7 No. 2 p.62 ~ p.68
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Inhibitory Effects of Essential Oil from Zanthoxylum coreanum Nakai on Melanin Production: Protection Against Re-Pigmentation after Laser Treatment
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Kim Ye-Na
Park Mi-Jin
Zheng Zhenlong
Lee Sung-Suk
Yoo Kwang-Ho
Kim Hee-Su
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Abstract
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Introduction: Zanthoxylum coreanum Nakai (ZCN) is a Korean lime tree that only grows in the southernmost areas of Korea, including Jeju Island. It has been used as both an herb in food and medicines for curing stomachache and skin disorders in Oriental medicine. Nevertheless, studies have yet to investigate the effects of ZCN on melanogenesis in the skin. This study was conducted to investigate the effects of ZCN essential oil on melanogenesis.
Materials and Methods: The essential oil was extracted from the fruits of ZCN by hydro-distillation at the National Institute of Forest Science. The viability of human dermal fibroblasts and B16F10 cells was determined by cell counting kit-8 assay. Mushroom tyrosinase activity assay and melanin assay were performed on B16F10 cells to evaluate the inhibitory effects of ZCN essential oil on melanogenesis. Also, gas chromatography (GC)-mass spectrometry (MS) analysis was performed to detect the components of ZCN essential oil.
Results: The ZCN essential oil inhibited human dermal fibroblast cell proliferation with an IC50 value of 0.06877% and B16F10 cell proliferation with an IC50 value of 0.01104%. Treatment with ZCN essential oil reduced tyrosinase activity in B16F10 cells, and the anti-melanogenic property was positively correlated with the concentration of ZCN essential oil. GC-MS analysis of ZCN essential oil identified β-Ocimene (24.48%), (-)-α-pinene (16.56%), 4-carvomenthenol (11.61%), and sabinene (10.81%) as the most abundant components.
Discussion: Our study showed that essential oil extracted from ZCN obtained from Jeju Island can be safely applied to the skin and may be useful as a potential skin-whitening ingredient in cosmeceuticals to prevent re-pigmentation or post-inflammatory hyperpigmentation after laser treatment.
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KEYWORD
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Zanthoxylum coreanum Nakai, Melanogenesis, B16F10 cells, Whitening
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